Characterization of superoxide dismutase in Streptococcus thermophilus

Characterization of superoxide dismutase in the rumen bacterium Streptococcus bovis

Superoxide dismutase (SOD) isoenzymes of the rumen bacterium Streptococcus bovis 4/1 were studied. Native PAGE showed a single band of Mn-SOD, unaffected by 10 mM cyanide or 5 mM hydrogen peroxide under both aerobic and anaerobic growth conditions. When the metals were removed from the growth medium by Chelex 100, the addition of manganese increased enzymatic activity, while addition of iron in...

Molecular characterization and functional analysis of the manganese-containing superoxide dismutase gene (sodA) from Streptococcus thermophilus AO54.

This report describes the isolation, sequencing, and functional analysis of the sodA gene, encoding Mn-superoxide dismutase, from Streptococcus thermophilus AO54. The gene was found to encode a 201 amino acid polypeptide with 88 and 83% identity to SodA from Streptococcus mutans and Streptococcus agalacticae, respectively. Primer extension analysis revealed a transcriptional start site 27 nucle...

Characterization of crystals of tetrameric manganese superoxide dismutase from Thermus thermophilus HB8.

The tetrameric manganese superoxide dismutase from the extreme thermophile Thermus thermophilus HB8 crystallizes in space group P41212 (or its enantiomorph) with a = b = 147.5 A, c = 55.9 A. The diffraction patterns extent to 1.4 A, and the crystals are very resistant to decay induced by x-irradiation. Measurements of the crystal density in Ficoll gradients are consistent with an asymmetric uni...

Superoxide dismutase Superoxide dismutase

Marker-free chromosomal integration of the manganese superoxide dismutase gene (sodA) from Streptococcus thermophilus into Lactobacillus gasseri.

A strategy for functional gene replacement in the chromosome of Lactobacillus gasseri is described. The phospho-beta-galactosidase II gene (lacII) was functionally replaced by the manganese superoxide dismutase (MnSOD) gene (sodA) from Streptococcus thermophilus, by adapting the insertional inactivation method described for lactobacilli [Russell, W.M. and Klaenhammer, T.R. 2001 Efficient system...